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Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in neuronal cells.

Anti-STIM2 Antibody

HPA036933
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSTIM2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-STIM2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57620
  • Target name
    STIM2
  • Target description
    stromal interaction molecule 2
  • Target synonyms
    stromal interaction molecule 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P246
  • Protein Name
    Stromal interaction molecule 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of STIM2 using anti-STIM2 antibody (A02345-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STIM2 antigen affinity purified polyclonal antibody (Catalog # A02345-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STIM2 at approximately 98,100 kDa. The expected band size for STIM2 is at 84 kDa.
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