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Western Blot Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, Mouse heart tissue All lanes: STIM2 antibody at 2.8ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 84, 94, 69 kDa Observed band size: 84, 69 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, Mouse heart tissue All lanes: STIM2 antibody at 2.8ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 84, 94, 69 kDa Observed band size: 84, 69 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, Mouse heart tissue All lanes: STIM2 antibody at 2.8ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 84, 94, 69 kDa Observed band size: 84, 69 kDa

STIM2 Antibody

CSB-PA022830LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSTIM2
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Overview

  • Supplier
    Cusabio
  • Product Name
    STIM2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57620
  • Target name
    STIM2
  • Target description
    stromal interaction molecule 2
  • Target synonyms
    stromal interaction molecule 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P246
  • Protein Name
    Stromal interaction molecule 2
  • Scientific Description
    Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Functions as a highly sensitive Ca(2+) sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca(2+)-influx. Regulates basal cytosolic and endoplasmic reticulum Ca(2+) concentrations. Upon mild variations of the endoplasmic reticulum Ca(2+) concentration, translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca(2+) release-activated Ca(2+) (CRAC) channels ORAI1, ORAI2 and ORAI3. May inhibit STIM1-mediated Ca(2+) influx.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of STIM2 using anti-STIM2 antibody (A02345-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STIM2 antigen affinity purified polyclonal antibody (Catalog # A02345-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STIM2 at approximately 98,100 kDa. The expected band size for STIM2 is at 84 kDa.
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Product group Antibodies
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