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ProductsBack/Anti-Sulphatase-2 [8G1]

Anti-Sulphatase-2 [8G1]

AB02941-10.0-BT
Absolute Antibody
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSULF2
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Sulphatase-2 [8G1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    This antibody was used for detection of Sulphatase-2 (SULF2), an extracellular sulfatase that acts on heparan sulfate proteoglycans and modulates multiple signaling pathways. The specificity of the monoclonal antibody was evaluated by ELISA, while sandwich ELISA was employed to detect the presence of SULF2 in blood and quantified its level.(PMID: 25444749) 8G1 reacted with the 75 kDa subunit of Sulf-2 and its 125 kDa proprotein precursor but not Sulf-1 by western blotting. s. The antibody was tested for its ability to revert the malignant phenotype of lung cancer cells (H292 cell line) in culture. The antibody markedly slow the growth of these cells on plastic dishes. 8G1 was tested for its effects on the growth of human lung cancer cells as xenograft tumors in immunocompromised host mice (nude mice), the antibody produced a marked inhibition of tumor size with time and in the final tumor weight (WO2012106192).
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    8G1
  • Gene ID55959
  • Target name
    SULF2
  • Target description
    sulfatase 2
  • Target synonyms
    extracellular sulfatase Sulf-2; HSULF-2
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDQ8IWU5
  • Protein Name
    Extracellular sulfatase Sulf-2
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SULF2 using anti-SULF2 antibody (A03903-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SULF2 antigen affinity purified polyclonal antibody (Catalog # A03903-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SULF2 at approximately 135 kDa. The expected band size for SULF2 is at 100,130-135 kDa.
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