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Western Blot Positive WB detected in: Hela whole cell lysate, Mouse brain tissue, Rat lung tissue All lanes: SULF2 antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 101 kDa Observed band size: 140 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, Mouse brain tissue, Rat lung tissue All lanes: SULF2 antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 101 kDa Observed band size: 140 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, Mouse brain tissue, Rat lung tissue All lanes: SULF2 antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 101 kDa Observed band size: 140 kDa

SULF2 Antibody

CSB-PA810283LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSULF2
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Overview

  • Supplier
    Cusabio
  • Product Name
    SULF2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55959
  • Target name
    SULF2
  • Target description
    sulfatase 2
  • Target synonyms
    extracellular sulfatase Sulf-2; HSULF-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IWU5
  • Protein Name
    Extracellular sulfatase Sulf-2
  • Scientific Description
    Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SULF2 using anti-SULF2 antibody (A03903-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SULF2 antigen affinity purified polyclonal antibody (Catalog # A03903-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SULF2 at approximately 135 kDa. The expected band size for SULF2 is at 100,130-135 kDa.
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