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Immunohistochemical staining of human cerebral cortex shows moderate to strong positivity in nuclear membrane in neuronal cells and glial cells.
Immunohistochemical staining of human cerebral cortex shows moderate to strong positivity in nuclear membrane in neuronal cells and glial cells.
Immunohistochemical staining of human cerebral cortex shows moderate to strong positivity in nuclear membrane in neuronal cells and glial cells.

Anti-SUN2 Antibody

HPA001209
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSUN2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SUN2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25777
  • Target name
    SUN2
  • Target description
    Sad1 and UNC84 domain containing 2
  • Target synonyms
    UNC84B, rab5IP, SUN domain-containing protein 2, Sad1 unc-84 domain protein 2, nuclear envelope protein, protein unc-84 homolog B, rab5-interacting protein, sad1/unc-84 protein-like 2, unc-84 homolog B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UH99
  • Protein Name
    SUN domain-containing protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SUN2 using anti-SUN2 antibody (A03291-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUN2 antigen affinity purified polyclonal antibody (Catalog # A03291-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUN2 at approximately 80 kDa. The expected band size for SUN2 is at 80 kDa.
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