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ProductsBack/SUN2 Recombinant Antibody
Lane 1: Rat Kidney; Probed with SUN2 (3G4) Monoclonal Antibody (bsm-54421R) at 1:2000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Rat Kidney; Probed with SUN2 (3G4) Monoclonal Antibody (bsm-54421R) at 1:2000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Rat Kidney; Probed with SUN2 (3G4) Monoclonal Antibody (bsm-54421R) at 1:2000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

SUN2 Recombinant Antibody

BSM-54421R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSUN2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    SUN2 (3G4) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IHC-P(1:200-400), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID25777
  • Target name
    SUN2
  • Target description
    Sad1 and UNC84 domain containing 2
  • Target synonyms
    nuclear envelope protein; protein unc-84 homolog B; rab5-interacting protein; rab5IP; Sad1 unc-84 domain protein 2; sad1/unc-84 protein-like 2; SUN domain-containing protein 2; unc-84 homolog B; UNC84B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UH99
  • Protein Name
    SUN domain-containing protein 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SUN2 using anti-SUN2 antibody (A03291-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUN2 antigen affinity purified polyclonal antibody (Catalog # A03291-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUN2 at approximately 80 kDa. The expected band size for SUN2 is at 80 kDa.
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