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Anti-SUPT6H Antibody

144-64378
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSUPT6H
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-SUPT6H Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID6830
  • Target name
    SUPT6H
  • Target description
    SPT6 homolog, histone chaperone and transcription elongation factor
  • Target synonyms
    emb-5; histone chaperone suppressor of Ty6; SPT6; SPT6H; suppressor of Ty 6 homolog; tat-cotransactivator 2 protein; tat-CT2 protein; transcription elongation factor SPT6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7KZ85
  • Protein Name
    Transcription elongation factor SPT6
  • Scientific Description
    SUPT6H pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Spt6/SUPT6H using anti-Spt6/SUPT6H antibody (A06484-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Spt6/SUPT6H antigen affinity purified polyclonal antibody (Catalog # A06484-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Spt6/SUPT6H at approximately 260 kDa. The expected band size for Spt6/SUPT6H is at 199 kDa.
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