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Immunohistochemical staining of human breast shows strong nuclear positivity in glandular cells and myoepithelial cells.
Immunohistochemical staining of human breast shows strong nuclear positivity in glandular cells and myoepithelial cells.
Immunohistochemical staining of human breast shows strong nuclear positivity in glandular cells and myoepithelial cells.

Anti-SUPT6H Antibody

HPA036382
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetSUPT6H
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SUPT6H
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6830
  • Target name
    SUPT6H
  • Target description
    SPT6 homolog, histone chaperone and transcription elongation factor
  • Target synonyms
    emb-5; histone chaperone suppressor of Ty6; SPT6; SPT6H; suppressor of Ty 6 homolog; tat-cotransactivator 2 protein; tat-CT2 protein; transcription elongation factor SPT6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7KZ85
  • Protein Name
    Transcription elongation factor SPT6
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Spt6/SUPT6H using anti-Spt6/SUPT6H antibody (A06484-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Spt6/SUPT6H antigen affinity purified polyclonal antibody (Catalog # A06484-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Spt6/SUPT6H at approximately 260 kDa. The expected band size for Spt6/SUPT6H is at 199 kDa.
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