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Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli.

Anti-SURF6 Antibody

HPA074622
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetSURF6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SURF6 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6838
  • Target name
    SURF6
  • Target description
    surfeit 6
  • Target synonyms
    RRP14, surfeit locus protein 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75683
  • Protein Name
    Surfeit locus protein 6
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of SURF6 using anti-SURF6 antibody (A11571-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SURF6 antigen affinity purified polyclonal antibody (Catalog # A11571-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SURF6 at approximately 45 kDa. The expected band size for SURF6 is at 41 kDa.
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