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Anti-Survivin Birc5 Rabbit Monoclonal Antibody

ORB547285
Biorbyt
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetBirc5
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Anti-Survivin Birc5 Rabbit Monoclonal Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    WB 1:500-1:2000IHC 1:50-1:200IP 1:30. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC, IP, WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    DGD-2
  • Conjugate
    Unconjugated
  • Gene ID11799
  • Target name
    Birc5
  • Target description
    baculoviral IAP repeat-containing 5
  • Target synonyms
    AAC-11, Api4, TIAP, survivin40, baculoviral IAP repeat-containing protein 5, apoptosis inhibitor 4, apoptosis inhibitor survivin, survivin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO70201
  • Protein Name
    Baculoviral IAP repeat-containing protein 5
  • Scientific Description
    Anti-Survivin Birc5 Rabbit Monoclonal Antibody. Tested in WB, IHC, IP applications. This antibody reacts with Mouse, Rat.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Western Blot Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate All lanes: Birc5 antibody at 5.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 17, 15, 5 kDa Observed band size: 17 kDa
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Figure 1. Western blot analysis of Survivin using anti-Survivin antibody (RP1052). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse SP2/0 whole cell lysates, Lane 3: mouse RAW264.7 whole cell lysates, Lane 4: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin antigen affinity purified polyclonal antibody (Catalog # RP1052) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin at approximately 16 kDa. The expected band size for Survivin is at 16 kDa.
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