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Immunohistochemical analysis of Survivin in a formalin-fixed, paraffin-embedded human brain tissue microarray using primary antibody dilution ratio of 1:2000. A. Differential expression of Survivin in brain tumors from six different patients. A1-A3, high magnification from A. A1, gemistocytic astrocytoma. A2, medulloblastoma. A3, choroid plexus papilloma. Hematoxylin-eosin counterstain.
Immunohistochemical analysis of Survivin in a formalin-fixed, paraffin-embedded human brain tissue microarray using primary antibody dilution ratio of 1:2000. A. Differential expression of Survivin in brain tumors from six different patients. A1-A3, high magnification from A. A1, gemistocytic astrocytoma. A2, medulloblastoma. A3, choroid plexus papilloma. Hematoxylin-eosin counterstain.
Immunohistochemical analysis of Survivin in a formalin-fixed, paraffin-embedded human brain tissue microarray using primary antibody dilution ratio of 1:2000. A. Differential expression of Survivin in brain tumors from six different patients. A1-A3, high magnification from A. A1, gemistocytic astrocytoma. A2, medulloblastoma. A3, choroid plexus papilloma. Hematoxylin-eosin counterstain.

Anti-Survivin birc5.1-a Antibody

A30032
Boster Bio
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetBIRC5
50 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Survivin birc5.1-a Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Gene ID332
  • Target name
    BIRC5
  • Target description
    baculoviral IAP repeat containing 5
  • Target synonyms
    API4, EPR-1, baculoviral IAP repeat-containing protein 5, apoptosis inhibitor 4, apoptosis inhibitor survivin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15392
  • Protein Name
    Baculoviral IAP repeat-containing protein 5
  • Scientific Description
    Boster Bio Anti-Survivin birc5.1-a Antibody (Catalog# A30032). Tested in IP, IHC, WB application(s). This antibody reacts with Human, Mouse, Rat.
  • UNSPSC
    12352203

References

  • Loss of cytoplasmic survivin expression is an independent predictor of poor prognosis in radically operated prostate cancer patients.
    Read more
  • Insight into the negative impact of ionic liquid: A cytotoxicity mechanism of 1-methyl-3-octylimidazolium bromide. Ma J et al., 2018 Nov, Environ Pollut
    Read more
  • Transcriptome analysis of Spodoptera frugiperda Sf9 cells reveals putative apoptosis-related genes and a preliminary apoptosis mechanism induced by azadirachtin.
    Read more
  • RASSF1A promotes apoptosis and suppresses the proliferation of ovarian cancer cells.
    Read more
  • The effects of STAT3 and Survivin silencing on the growth of human bladder carcinoma cells.
    Read more
  • Reactive oxygen species mediate isoalantolactone-induced apoptosis in human prostate cancer cells.
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  • Expression of survivin and human telomerase reverse transcriptase in extramammary Pagets disease.
    Read more
  • Knockdown of survivin and upregulation of p53 gene expression by small interfering RNA induces apoptosis in human gastric carcinoma cell line SGC-823.
    Read more
  • Prognostic significance of Survivin and CD44v6 in laryngeal cancer surgical margins.
    Read more

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Figure 1. Western blot analysis of Survivin using anti-Survivin antibody (A00379). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin antigen affinity purified polyclonal antibody (Catalog # A00379) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin at approximately 16 kDa. The expected band size for Survivin is at 16 kDa.
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