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Anti-SYGP1 SYNGAP1 Antibody

A04862
Boster Bio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSYNGAP1
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-SYGP1 SYNGAP1 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID8831
  • Target name
    SYNGAP1
  • Target description
    synaptic Ras GTPase activating protein 1
  • Target synonyms
    MRD5, RASA1, RASA5, SYNGAP, ras/Rap GTPase-activating protein SynGAP, Ras GTPase-activating protein SynGAP, Ras/Rap GTPase-activating protein 1, neuronal RasGAP, synaptic Ras GTPase activating protein 1 homolog, synaptic Ras GTPase activating protein, 135kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96PV0
  • Protein Name
    Ras/Rap GTPase-activating protein SynGAP
  • Scientific Description
    Boster Bio Anti-SYGP1 SYNGAP1 Antibody catalog # A04862. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SYNGAP1 using anti-SYNGAP1 antibody (A04862-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYNGAP1 antigen affinity purified polyclonal antibody (Catalog # A04862-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYNGAP1 at approximately 148 kDa. The expected band size for SYNGAP1 is at 148 kDa.
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