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Anti-TAF12 Antibody

144-05421
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetTAF12
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-TAF12 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6883
  • Target name
    TAF12
  • Target description
    TATA-box binding protein associated factor 12
  • Target synonyms
    TAF2J, TAFII20, transcription initiation factor TFIID subunit 12, TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa, TAFII-20/TAFII-15, TAFII20/TAFII15, TATA box binding protein (TBP)-associated factor, RNA polymerase II, J, 20kD, transcription initiation factor TFIID 20/15 kDa subunits
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16514
  • Protein Name
    Transcription initiation factor TFIID subunit 12
  • Scientific Description
    TAF12 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TAF12 using anti-TAF12 antibody (A06944-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-937 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAF12 antigen affinity purified polyclonal antibody (Catalog # A06944-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAF12 at approximately 22KD. The expected band size for TAF12 is at 18KD.
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