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Immunohistochemical staining of human colon shows moderate nuclear and cytplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate nuclear and cytplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate nuclear and cytplasmic positivity in glandular cells.

Anti-TAF4 Antibody

HPA008599
Atlas Antibodies
ApplicationsChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTAF4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TAF4 Antibody
  • Delivery Days Customer
    4
  • Applications
    ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6874
  • Target name
    TAF4
  • Target description
    TATA-box binding protein associated factor 4
  • Target synonyms
    MRD73, TAF(II)130, TAF(II)135, TAF2C, TAF2C1, TAF4A, TAFII-130, TAFII-135, TAFII130, TAFII135, transcription initiation factor TFIID subunit 4, RNA polymerase II TBP-associated factor subunit C, TAF4 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135kDa, TAF4A RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135 kD, TATA box binding protein (TBP)-associated factor, RNA polymerase II, C1, 130kD, TBP-associated factor 4, transcription initiation factor TFIID 130 kDa subunit, transcription initiation factor TFIID 135 kD subunit, transcription initiation factor TFIID 135 kDa subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00268
  • Protein Name
    Transcription initiation factor TFIID subunit 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of TAF4 using anti-TAF4 antibody (A05511-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAF4 antigen affinity purified polyclonal antibody (Catalog # A05511-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAF4 at approximately 135KD. The expected band size for TAF4 is at 110KD.
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