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TAF4/TAF4B Antibody

CSB-PA050203
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetTAF4
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Overview

  • Supplier
    Cusabio
  • Product Name
    TAF4/TAF4B Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6874
  • Target name
    TAF4
  • Target description
    TATA-box binding protein associated factor 4
  • Target synonyms
    MRD73, TAF(II)130, TAF(II)135, TAF2C, TAF2C1, TAF4A, TAFII-130, TAFII-135, TAFII130, TAFII135, transcription initiation factor TFIID subunit 4, RNA polymerase II TBP-associated factor subunit C, TAF4 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135kDa, TAF4A RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135 kD, TATA box binding protein (TBP)-associated factor, RNA polymerase II, C1, 130kD, TBP-associated factor 4, transcription initiation factor TFIID 130 kDa subunit, transcription initiation factor TFIID 135 kD subunit, transcription initiation factor TFIID 135 kDa subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00268
  • Protein Name
    Transcription initiation factor TFIID subunit 4
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TAF4 using anti-TAF4 antibody (A05511-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAF4 antigen affinity purified polyclonal antibody (Catalog # A05511-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAF4 at approximately 135KD. The expected band size for TAF4 is at 110KD.
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