Figure 1. Western blot analysis of TAF8/TBN using anti-TAF8/TBN antibody (A11118-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse lung tissue lysates, Lane 9: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAF8/TBN antigen affinity purified polyclonal antibody (Catalog # A11118-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAF8/TBN at approximately 34 kDa. The expected band size for TAF8/TBN is at 34 kDa.
Figure 1. Western blot analysis of TAF8/TBN using anti-TAF8/TBN antibody (A11118-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse lung tissue lysates, Lane 9: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAF8/TBN antigen affinity purified polyclonal antibody (Catalog # A11118-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAF8/TBN at approximately 34 kDa. The expected band size for TAF8/TBN is at 34 kDa.
Anti-TAF8/TBN Antibody Picoband(r)
A11118-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
TargetTAF8
Overview
- SupplierBoster Bio
- Product NameAnti-TAF8/TBN Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID129685
- Target nameTAF8
- Target descriptionTATA-box binding protein associated factor 8
- Target synonymsII, NEDMLHB, TAF, TAF(II)43, TAFII-43, TAFII43, TBN, transcription initiation factor TFIID subunit 8, TAF8 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 43kDa, TATA box binding protein (TBP)-associated factor, RNA polymerase II, A, 45/50kDa, TBP-associated factor 43 kDa, TBP-associated factor 8, TBP-associated factor TAFII43, TBP-associated factor, RNA polymerase II, 43 kD, hTAFII43, protein taube nuss, taube nuss homolog, transcription initiation factor TFIID 43 kDa subunit
- HostRabbit
- IsotypeIgG
- Protein IDQ7Z7C8
- Protein NameTranscription initiation factor TFIID subunit 8
- Scientific DescriptionBoster Bio Anti-TAF8/TBN Antibody Picoband® catalog # A11118-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
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