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TAF8 Antibody

PACO53870
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTAF8
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Overview

  • Supplier
    Assay Genie
  • Product Name
    TAF8 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID129685
  • Target name
    TAF8
  • Target description
    TATA-box binding protein associated factor 8
  • Target synonyms
    II, NEDMLHB, TAF, TAF(II)43, TAFII-43, TAFII43, TBN, transcription initiation factor TFIID subunit 8, TAF8 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 43kDa, TATA box binding protein (TBP)-associated factor, RNA polymerase II, A, 45/50kDa, TBP-associated factor 43 kDa, TBP-associated factor 8, TBP-associated factor TAFII43, TBP-associated factor, RNA polymerase II, 43 kD, hTAFII43, protein taube nuss, taube nuss homolog, transcription initiation factor TFIID 43 kDa subunit
  • Host
    Rabbit
  • Protein IDQ7Z7C8
  • Protein Name
    Transcription initiation factor TFIID subunit 8
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Reactivity Supplier
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of TAF8/TBN using anti-TAF8/TBN antibody (A11118-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse lung tissue lysates, Lane 9: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAF8/TBN antigen affinity purified polyclonal antibody (Catalog # A11118-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAF8/TBN at approximately 34 kDa. The expected band size for TAF8/TBN is at 34 kDa.
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