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Anti-TAGAP (Center) Antibody

102-20676
RayBiotech
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTAGAP
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-TAGAP (Center) Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB22008
  • Gene ID117289
  • Target name
    TAGAP
  • Target description
    T cell activation RhoGTPase activating protein
  • Target synonyms
    ARHGAP47; FKSG15; IDDM21; TAGAP1; T-cell activation Rho GTPase-activating protein
  • Host
    Rabbit
  • Protein IDQ8N103
  • Protein Name
    T-cell activation Rho GTPase-activating protein
  • Scientific Description
    Rabbit Anti-TAGAP (Center) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TAGAP using anti-TAGAP antibody (M09403). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAGAP antigen affinity purified monoclonal antibody (Catalog # M09403) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAGAP at approximately 72 kDa. The expected band size for TAGAP is at 81 kDa.
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