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Immunofluorescent staining of human cell line HaCaT shows localization to cytosol.
Immunofluorescent staining of human cell line HaCaT shows localization to cytosol.
Immunofluorescent staining of human cell line HaCaT shows localization to cytosol.

Anti-TAGAP Antibody

HPA039982
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetTAGAP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TAGAP
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID117289
  • Target name
    TAGAP
  • Target description
    T cell activation RhoGTPase activating protein
  • Target synonyms
    ARHGAP47; FKSG15; IDDM21; TAGAP1; T-cell activation Rho GTPase-activating protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N103
  • Protein Name
    T-cell activation Rho GTPase-activating protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TAGAP using anti-TAGAP antibody (M09403). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAGAP antigen affinity purified monoclonal antibody (Catalog # M09403) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAGAP at approximately 72 kDa. The expected band size for TAGAP is at 81 kDa.
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Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in glial cells.
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