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Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to nucleoplasm.

Anti-TAL1 Antibody

HPA073983
Atlas Antibodies
ApplicationsChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetTAL1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TAL1
  • Delivery Days Customer
    4
  • Applications
    ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6886
  • Target name
    TAL1
  • Target description
    TAL bHLH transcription factor 1, erythroid differentiation factor
  • Target synonyms
    bHLHa17; class A basic helix-loop-helix protein 17; SCL; stem cell protein; tal-1; tal-1 product; T-cell acute lymphocytic leukemia 1; T-cell acute lymphocytic leukemia protein 1; T-cell leukemia/lymphoma protein 5; TCL5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17542
  • Protein Name
    T-cell acute lymphocytic leukemia protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of Tal1 using anti-Tal1 antibody (A00944-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U20S cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tal1 antigen affinity purified polyclonal antibody (Catalog # A00944-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tal1 at approximately 39KD. The expected band size for Tal1 is at 34KD.
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