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Western Blot Positive WB detected in: Raji whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, 293 whole cell lysate All lanes: TAL1 antibody at 5.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 32, 17 kDa Observed band size: 35 kDa
Western Blot Positive WB detected in: Raji whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, 293 whole cell lysate All lanes: TAL1 antibody at 5.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 32, 17 kDa Observed band size: 35 kDa
Western Blot Positive WB detected in: Raji whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, 293 whole cell lysate All lanes: TAL1 antibody at 5.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 32, 17 kDa Observed band size: 35 kDa

TAL1 Antibody

CSB-PA023110LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetTAL1
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Overview

  • Supplier
    Cusabio
  • Product Name
    TAL1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6886
  • Target name
    TAL1
  • Target description
    TAL bHLH transcription factor 1, erythroid differentiation factor
  • Target synonyms
    SCL, TCL5, bHLHa17, tal-1, T-cell acute lymphocytic leukemia protein 1, T-cell acute lymphocytic leukemia 1, T-cell leukemia/lymphoma protein 5, class A basic helix-loop-helix protein 17, stem cell protein, tal-1 product
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17542
  • Protein Name
    T-cell acute lymphocytic leukemia protein 1
  • Scientific Description
    Implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation. Serves as a positive regulator of erythroid differentiation (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Tal1 using anti-Tal1 antibody (A00944-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U20S cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tal1 antigen affinity purified polyclonal antibody (Catalog # A00944-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tal1 at approximately 39KD. The expected band size for Tal1 is at 34KD.
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