Anti-Talin 1 [8D4]

Absolute Antibody

Anti-Talin 1 [8D4]

9

The antibody detected the N-terminal region of talin1 by western blot analysis (Praekelt et al., 2012; PMID: 22306379 and Martel et al., 2001; PMID: 11279249). However, it also detected the equivalent region of talin2, albeit to a lesser degree (Praekelt et al., 2012; PMID: 22306379). This antibody was used for detection of talin expression on HeLa/AT22, AT21, wild-type HeLa cells or pMEP4-transfected HeLa cells cells by flow cytometry (Albigs-Rizo et al., 1995; PMID: 7593292). lmmunocytochemistry using the antibody showed patterns of talin expression within DRG growth cones (Sydor et al., 1996; PMID: 8794861). Indirect immunofluorescence staining of cultured fibroblasts (rat, human, chicken and xenopus embryo fibroblasts) was tested with the antibody. In all the samples the focal adhesions were stained with the antibody (Otey et al. 1990; PMID: 1690175). Talin was immunoprecipitated from MDA-MB-231 cell lysates using the antibody (Beaty et al., 2014; PMID: 24891603).

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry

Research Use Only

Monoclonal

8D4

TLN1

talin 1

ILWEQ; talin-1; TLN

Mouse

IgG1

Talin-1

-20°C,2°C to 8°C

12352203