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Anti-TLN1 Antibody

Anti-TLN1 Antibody

A97884
Antibodies.com
ApplicationsWestern Blot, ELISA
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-TLN1 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to TLN1.
  • UNSPSC
    12352203

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Figure 2. IHC analysis of Talin 1 using anti-Talin 1 antibody (A02859-1). Talin 1 was detected in paraffin-embedded section of human Lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2microg/ml rabbit anti-Talin 1 Antibody (A02859-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer normal goat serum at 37°C for 30min; Antibody incubation with Talin Polyclonal Antibody, Unconjugated (bs-3619R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
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