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Anti-TAP2 Antibody

144-01610
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTAP2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-TAP2 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6891
  • Target name
    TAP2
  • Target description
    transporter 2, ATP binding cassette subfamily B member
  • Target synonyms
    ABC18, ABCB3, APT2, D6S217E, MHC1D2, PSF-2, PSF2, RING11, antigen peptide transporter 2, ABC transporter, MHC 2, ATP-binding cassette, sub-family B (MDR/TAP), member 3, peptide supply factor 2, peptide transporter PSF2, peptide transporter involved in antigen processing 2, really interesting new gene 11 protein, transporter 2, ABC (ATP binding cassette), transporter 2, ATP-binding cassette, sub-family B (MDR/TAP)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ03519
  • Protein Name
    Antigen peptide transporter 2
  • Scientific Description
    TAP2 Polyclonal Antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TAP2 using anti-TAP2 antibody (PB9824). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAP2 antigen affinity purified polyclonal antibody (Catalog # PB9824) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAP2 at approximately 87 kDa. The expected band size for TAP2 is at 87 kDa.
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