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ProductsBack/Anti-TDP43 Rabbit Monoclonal Antibody
All lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.

Anti-TDP43 Rabbit Monoclonal Antibody

M01001
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat, Zebra Fish
TargetTARDBP
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-TDP43 Rabbit Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ABAB-20
  • Gene ID23435
  • Target name
    TARDBP
  • Target description
    TAR DNA binding protein
  • Target synonyms
    ALS10, TDP-43, TAR DNA-binding protein 43, TAR DNA-binding protein-43
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13148
  • Protein Name
    TAR DNA-binding protein 43
  • Scientific Description
    Boster Bio Anti-TDP43 TARDBP Rabbit Monoclonal Antibody catalog # M01001. Tested in WB, IHC, ICC, IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat, Zebrafish.
  • Reactivity
    Human, Mouse, Rat, Zebra Fish
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Zhang Y, Yang C, Yang S, et al. MiRNA-27a decreases ultraviolet B irradiation-induced cell damage. J Cell Biochem. 2020,121(2):1032-1038. doi: 10.1002/jcb.29337
    Read this paper

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of TDP-43/TARDBP using anti-TDP-43/TARDBP antibody (A01001-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse pancrease lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TDP-43/TARDBP antigen affinity purified polyclonal antibody (Catalog # A01001-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TDP-43/TARDBP at approximately 43 kDa. The expected band size for TDP-43/TARDBP is at 45 kDa.
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Western Blot Positive WB detected in: K562 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, HL60 whole cell lyasate, MCF-7 whole cell lysate, HepG2 whole cell lysate, SH-SY5Y whole cell lysate All lanes: TARDBP antibody at 2.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45, 32 kDa Observed band size: 45 kDa
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