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Immunohistochemical staining of human placenta shows nuclear positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows nuclear positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows nuclear positivity in trophoblastic cells.

Anti-TEAD3 Antibody

HPA028906
Atlas Antibodies
ApplicationsChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTEAD3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TEAD3 Antibody
  • Delivery Days Customer
    4
  • Applications
    ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7005
  • Target name
    TEAD3
  • Target description
    TEA domain transcription factor 3
  • Target synonyms
    DTEF-1, ETFR-1, TEAD-3, TEAD5, TEF-5, TEF5, transcriptional enhancer factor TEF-5, TEA domain family member 3, TEA domain family member 5, transcriptional enhancer factor 5, transcriptional enhancer factor TEF-5 (DTEF-1)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99594
  • Protein Name
    Transcriptional enhancer factor TEF-5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of TEAD3 using anti-TEAD3 antibody (A07748-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TEAD3 antigen affinity purified polyclonal antibody (Catalog # A07748-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TEAD3 at approximately 54 kDa. The expected band size for TEAD3 is at 49 kDa.
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