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TEAD3 Antibody

CAB7454
Assay Genie
ReactivityHuman
Product group Antibodies
TargetTEAD3
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Overview

  • Supplier
    Assay Genie
  • Product Name
    TEAD3 Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC,IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID7005
  • Target name
    TEAD3
  • Target description
    TEA domain transcription factor 3
  • Target synonyms
    DTEF-1, ETFR-1, TEAD-3, TEAD5, TEF-5, TEF5, transcriptional enhancer factor TEF-5, TEA domain family member 3, TEA domain family member 5, transcriptional enhancer factor 5, transcriptional enhancer factor TEF-5 (DTEF-1)
  • Host
    Rabbit
  • Protein IDQ99594
  • Protein Name
    Transcriptional enhancer factor TEF-5
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TEAD3 using anti-TEAD3 antibody (A07748-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TEAD3 antigen affinity purified polyclonal antibody (Catalog # A07748-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TEAD3 at approximately 54 kDa. The expected band size for TEAD3 is at 49 kDa.
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