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Anti-TGF beta1 [SL15S]

AB03786-10.0
Absolute Antibody
ApplicationsELISA, Neutralisation/Blocking
Product group Antibodies
TargetTGFB1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-TGF beta1 [SL15S]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The specificity of the original format of the antibody was confirmed by ELISA analysis. The ability of the scFv fragment to recognise TGFbeta1 and neutralise binding of TGFbeta1 to A549 cells was measured in the radioreceptor assay. The scFv fragment was a highly potent, neutralising antibody for TGFbeta1, with IC50 values in the range 0.03 to 0 1 nM. In particular, the scFv fragment could neutralise active, but not latent, TGFbeta1 (US7151169B2).
  • Applications
    ELISA, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SL15S
  • Gene ID7040
  • Target name
    TGFB1
  • Target description
    transforming growth factor beta 1
  • Target synonyms
    CED; DPD1; IBDIMDE; LAP; latency-associated peptide; prepro-transforming growth factor beta-1; TGFB; TGFbeta; TGF-beta1; TGF-beta-1; transforming growth factor beta1; transforming growth factor beta-1 proprotein
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP01137
  • Protein Name
    Transforming growth factor beta-1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. IHC analysis of TGF Beta 1/TGFB1 using anti-TGF Beta 1/TGFB1 antibody (A00019-2). TGF Beta 1/TGFB1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 microg/ml rabbit anti-TGF Beta 1/TGFB1 Antibody (A00019-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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