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Immunohistochemical staining of human thymus shows strong cytoplasmic positivity in cortical cells.
Immunohistochemical staining of human thymus shows strong cytoplasmic positivity in cortical cells.
Immunohistochemical staining of human thymus shows strong cytoplasmic positivity in cortical cells.

Anti-THEMIS Antibody

HPA031425
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetTHEMIS
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-THEMIS
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID387357
  • Target name
    THEMIS
  • Target description
    thymocyte selection associated
  • Target synonyms
    C6orf190; C6orf207; GASP; GRB2-associated protein; protein THEMIS; signaling phosphoprotein specific for T cells; SPOT; thymocyte selection pathway associated; thymocyte-expressed molecule involved in selection; TSEPA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N1K5
  • Protein Name
    Protein THEMIS
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Jurkat whole cell lysate, K562 whole cell lysate All lanes: THEMIS antibody at 7.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 74, 70, 63, 78 kDa Observed band size: 74, 70 kDa
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Figure 1. Western blot analysis of THEMIS using anti-THEMIS antibody (A07854-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THEMIS antigen affinity purified polyclonal antibody (Catalog # A07854-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for THEMIS at approximately 73 kDa. The expected band size for THEMIS is at 73 kDa.
Product group Antibodies
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Anti-THEMIS Antibody Picoband(r)A07854-3-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetTHEMIS
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Product group Antibodies
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THEMIS antibody (FITC)GTX47850
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
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Product group Antibodies
RayBiotech
Anti-Mouse THEMIS (C-term) Antibody102-20777
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
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Product group Antibodies
Bethyl Laboratories
Rabbit anti-THEMIS Antibody, Affinity PurifiedA305-277A
ApplicationsImmunoPrecipitation, Western Blot
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Protein IDQ8N1K5
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THEMIS / SPOT AntibodyLS-C674606
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