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THEMIS Antibody

PACO57384
Assay Genie
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
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Product group Antibodies
TargetTHEMIS
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Overview

  • Supplier
    Assay Genie
  • Product Name
    THEMIS Antibody (PACO57384)
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID387357
  • Target name
    THEMIS
  • Target description
    thymocyte selection associated
  • Target synonyms
    C6orf190; C6orf207; GASP; GRB2-associated protein; protein THEMIS; signaling phosphoprotein specific for T cells; SPOT; thymocyte selection pathway associated; thymocyte-expressed molecule involved in selection; TSEPA
  • Host
    Rabbit
  • Protein IDQ8N1K5
  • Protein Name
    Protein THEMIS
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Jurkat whole cell lysate, K562 whole cell lysate All lanes: THEMIS antibody at 7.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 74, 70, 63, 78 kDa Observed band size: 74, 70 kDa
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Figure 1. Western blot analysis of THEMIS using anti-THEMIS antibody (A07854-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THEMIS antigen affinity purified polyclonal antibody (Catalog # A07854-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for THEMIS at approximately 73 kDa. The expected band size for THEMIS is at 73 kDa.
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