More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-TIGIT [4D4]

Anti-TIGIT [4D4]

Ab00973-2.3
Absolute Antibody
ApplicationsFlow Cytometry, Other Application
Product group Antibodies
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-TIGIT [4D4]
  • Delivery Days Customer
    7
  • Application Supplier Note
    4D4 is an agonistic anti-TIGIT antibody, and addition of plate bound 4D4 to anti-CD3/anti-CD28 stimulated T cells was shown to inhibit their proliferation (Joller 2011). Further, 4D4 has been used in order to identify genes that are downstream of TIGIT signaling in Tregs, as TIGIT engagement might modulate the Treg phenotype in tumor tissue (Liu 2016).
  • Applications
    Flow Cytometry, Other Application
  • Applications Supplier
    Agonist; Fc
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4D4
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP86176
  • Protein Name
    T-cell immunoreceptor with Ig and ITIM domains
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Hamster format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Mouse
  • Reactivity Supplier Note
    Armenian hamsters were immunized with recombinant mouse TIGIT tetramers by a combination of s.c. and food pad immunization and booster injections. Draining lymph nodes were fused with Sp2/0-Ag14 cells, selected in HAT (hypoxanthine/aminopterin/thymidine) medium and supernatants were screened for specificity by ELISA and flow cytometry using TIGIT-transfectants.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Absolute Antibody
Anti-TIGIT [4D4]AB00973-2.3-VXS
ApplicationsFlow Cytometry, Other Application
  • SizePrice
Figure 1. Western blot analysis of Tigit using anti-Tigit antibody (A01962-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat plasma lysates, Lane 3: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tigit antigen affinity purified polyclonal antibody (Catalog # A01962-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tigit at approximately 30KD. The expected band size for Tigit is at 30KD.
Product group Antibodies
Boster Bio
Anti-Tigit Antibody Picoband(r)A01962-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetTigit
  • SizePrice
FACS analysis of mouse C57Bl/6 splenocytes using GTX01496-07 TIGIT antibody [1G9] (APC). Right panel : co-stained with TIGIT antibody [1G9] (APC) and Mouse CD4 antibody (FITC) Left panel : co-stained with isotype control and Mouse CD4 antibody (FITC) antibody amount : 0.06 microg (5 microl)
Product group Antibodies
References
GeneTex
TIGIT antibody [1G9] (APC)GTX01496-07
ApplicationsFlow Cytometry
TargetTigit
  • SizePrice
Product group Antibodies
Sino Biological
Anti-TIGIT Antibody, Rabbit Polyclonal10917-T16
ApplicationsELISA
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-TIGIT Recombinant Monoclonal Antibody [BLR235K]A700-235
ApplicationsWestern Blot
TargetTigit
  • SizePrice