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ProductsBack/TIGIT antibody [1G9] (APC)
FACS analysis of mouse C57Bl/6 splenocytes using GTX01496-07 TIGIT antibody [1G9] (APC). Right panel : co-stained with TIGIT antibody [1G9] (APC) and Mouse CD4 antibody (FITC) Left panel : co-stained with isotype control and Mouse CD4 antibody (FITC) antibody amount : 0.06 microg (5 microl)
FACS analysis of mouse C57Bl/6 splenocytes using GTX01496-07 TIGIT antibody [1G9] (APC). Right panel : co-stained with TIGIT antibody [1G9] (APC) and Mouse CD4 antibody (FITC) Left panel : co-stained with isotype control and Mouse CD4 antibody (FITC) antibody amount : 0.06 microg (5 microl)
FACS analysis of mouse C57Bl/6 splenocytes using GTX01496-07 TIGIT antibody [1G9] (APC). Right panel : co-stained with TIGIT antibody [1G9] (APC) and Mouse CD4 antibody (FITC) Left panel : co-stained with isotype control and Mouse CD4 antibody (FITC) antibody amount : 0.06 microg (5 microl)

TIGIT antibody [1G9] (APC)

GTX01496-07
GeneTex
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityMouse
TargetTigit
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Overview

  • Supplier
    GeneTex
  • Product Name
    TIGIT antibody [1G9] (APC)
  • Delivery Days Customer
    9
  • Application Supplier Note
    *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1G9
  • Concentration
    0.2 mg/ml
  • Conjugate
    APC (Allophycocyanin)
  • Gene ID100043314
  • Target name
    Tigit
  • Target description
    T cell immunoreceptor with Ig and ITIM domains
  • Target synonyms
    Vstm3, T-cell immunoreceptor with Ig and ITIM domains, V-set and transmembrane domain containing 3, V-set and transmembrane domain-containing protein 3
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Joller N, Peters A, Anderson AC, et al. Immune checkpoints in central nervous system autoimmunity. Immunol Rev. 2012,248(1):122-39. doi: 10.1111/j.1600-065X.2012.01136.x
    Read this paper
  • Joller N, Hafler JP, Brynedal B, et al. Cutting edge: TIGIT has T cell-intrinsic inhibitory functions. J Immunol. 2011,186(3):1338-42. doi: 10.4049/jimmunol.1003081
    Read this paper

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FACS analysis of mouse C57Bl/6 splenocytes using GTX01496-08 TIGIT antibody [1G9] (PE). Right panel : co-stained with TIGIT antibody [1G9] (PE) and Mouse CD4 antibody (FITC) Left panel : co-stained with isotype control and Mouse CD4 antibody (FITC) antibody amount : 0.25 microg (5 microl)
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Figure 1. Western blot analysis of Tigit using anti-Tigit antibody (A01962-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat plasma lysates, Lane 3: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tigit antigen affinity purified polyclonal antibody (Catalog # A01962-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tigit at approximately 30KD. The expected band size for Tigit is at 30KD.
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