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Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Anti-TJAP1 Antibody

HPA030164
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTJAP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TJAP1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID93643
  • Target name
    TJAP1
  • Target description
    tight junction associated protein 1
  • Target synonyms
    PILT, TJP4, tight junction-associated protein 1, protein incorporated later into tight junctions, tight junction associated protein 1 (peripheral), tight junction protein 4 (peripheral)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5JTD0
  • Protein Name
    Tight junction-associated protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of TJAP1 using anti-TJAP1 antibody (A12692-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TJAP1 antigen affinity purified polyclonal antibody (Catalog # A12692-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TJAP1 at approximately 86 kDa. The expected band size for TJAP1 is at 62 kDa.
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