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Immunohistochemical staining of human placenta shows strong cytoplasmic positivity with a granular pattern in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity with a granular pattern in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity with a granular pattern in trophoblastic cells.

Anti-TJAP1 Antibody

HPA030165
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTJAP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TJAP1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID93643
  • Target name
    TJAP1
  • Target description
    tight junction associated protein 1
  • Target synonyms
    PILT, TJP4, tight junction-associated protein 1, protein incorporated later into tight junctions, tight junction associated protein 1 (peripheral), tight junction protein 4 (peripheral)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5JTD0
  • Protein Name
    Tight junction-associated protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of TJAP1 using anti-TJAP1 antibody (A12692-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TJAP1 antigen affinity purified polyclonal antibody (Catalog # A12692-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TJAP1 at approximately 86 kDa. The expected band size for TJAP1 is at 62 kDa.
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