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Immunohistochemical staining of human spleen shows moderate to strong cytoplasmic positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows moderate to strong cytoplasmic positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows moderate to strong cytoplasmic positivity in cells in red pulp.

Anti-TLR4 Antibody

HPA049174
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetTLR4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TLR4
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7099
  • Target name
    TLR4
  • Target description
    toll like receptor 4
  • Target synonyms
    ARMD10; CD284; homolog of Drosophila toll; hToll; TLR-4; TOLL; toll like receptor 4 protein; toll-like receptor 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00206
  • Protein Name
    Toll-like receptor 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TLR4 using anti-TLR4 antibody (A00017-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR4 antigen affinity purified polyclonal antibody (Catalog # A00017-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR4 at approximately 100 kDa. The expected band size for TLR4 is at 96 kDa.
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