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ProductsBack/Goat anti-TLR4 / CD284

Goat anti-TLR4 / CD284

EB09441
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTLR4
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-TLR4 / CD284 Antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 10microg/ml Flow Cytometry: Flow cytometric analysis of U937 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID7099
  • Target name
    TLR4
  • Target description
    toll like receptor 4
  • Target synonyms
    ARMD10, CD284, TLR-4, TOLL, toll-like receptor 4, hToll, homolog of Drosophila toll, toll like receptor 4 protein
  • Host
    Goat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TLR4 using anti-TLR4 antibody (A00017-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR4 antigen affinity purified polyclonal antibody (Catalog # A00017-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR4 at approximately 100 kDa. The expected band size for TLR4 is at 96 kDa.
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