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Immunohistochemical staining of human cerebellum shows moderate membrane positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows moderate membrane positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows moderate membrane positivity in Purkinje cells.

Anti-TMEM240 Antibody

HPA066721
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTMEM240
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TMEM240 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID339453
  • Target name
    TMEM240
  • Target description
    transmembrane protein 240
  • Target synonyms
    C1orf70, SCA21, transmembrane protein 240, transmembrane protein C1orf70
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5SV17
  • Protein Name
    Transmembrane protein 240
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TMEM240 using anti-TMEM240 antibody (A14889). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: NIH3T3 whole Cell lysates, Lane 3: COLO320 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM240 antigen affinity purified polyclonal antibody (Catalog # A14889) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM240 at approximately 19KD. The expected band size for TMEM240 is at 19KD.
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