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TMEM240 / C1orf70 Antibody

LS-C501038
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
ReactivityHuman
TargetTMEM240
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TMEM240 / C1orf70 Antibody
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, ELISA
  • Applications Supplier
    ELISA, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID339453
  • Target name
    TMEM240
  • Target description
    transmembrane protein 240
  • Target synonyms
    C1orf70, SCA21, transmembrane protein 240, transmembrane protein C1orf70
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TMEM240 using anti-TMEM240 antibody (A14889). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: NIH3T3 whole Cell lysates, Lane 3: COLO320 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM240 antigen affinity purified polyclonal antibody (Catalog # A14889) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM240 at approximately 19KD. The expected band size for TMEM240 is at 19KD.
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