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Immunohistochemical staining of human pancreas shows moderate to strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows moderate to strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows moderate to strong cytoplasmic positivity in exocrine glandular cells.

Anti-TMEM97 Antibody

HPA044795
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTMEM97
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TMEM97 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27346
  • Target name
    TMEM97
  • Target description
    transmembrane protein 97
  • Target synonyms
    MAC30, S2R, sigma2R, sigma intracellular receptor 2, meningioma-associated protein 30, sigma-2 receptor, sigma2 receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5BJF2
  • Protein Name
    Sigma intracellular receptor 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Immunohistochemistry analysis in human pancreas and salivary gland tissues using Anti-TMEM97 antibody. Corresponding TMEM97 RNA-seq data are presented for the same tissues.
Product group Antibodies
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Figure 1. Western blot analysis of TMEM97 using anti-TMEM97 antibody (A08336-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM97 antigen affinity purified polyclonal antibody (Catalog # A08336-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM97 at approximately 23 kDa. The expected band size for TMEM97 is at 21 kDa.
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ReactivityHuman, Rat
TargetTMEM97
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