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MAC30 / TMEM97 Antibody (aa105-155)

LS-C143201
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse
TargetTMEM97
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MAC30 / TMEM97 Antibody (aa105-155)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    ICC (1:200), IF (1:50 - 1:200), WB (2 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID27346
  • Target name
    TMEM97
  • Target description
    transmembrane protein 97
  • Target synonyms
    MAC30, S2R, sigma2R, sigma intracellular receptor 2, meningioma-associated protein 30, sigma-2 receptor, sigma2 receptor
  • Host
    Rabbit
  • Reactivity
    Human, Monkey, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TMEM97 using anti-TMEM97 antibody (A08336-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM97 antigen affinity purified polyclonal antibody (Catalog # A08336-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM97 at approximately 23 kDa. The expected band size for TMEM97 is at 21 kDa.
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