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Immunohistochemical staining of human testis cancer (embryonal carcinoma) shows moderate membranous positivity in tumor cells.
Immunohistochemical staining of human testis cancer (embryonal carcinoma) shows moderate membranous positivity in tumor cells.
Immunohistochemical staining of human testis cancer (embryonal carcinoma) shows moderate membranous positivity in tumor cells.

Anti-TNFRSF8 Antibody

AMAB91800
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTNFRSF8
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TNFRSF8
  • Delivery Days Customer
    12
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL12632
  • Conjugate
    Unconjugated
  • Gene ID943
  • Target name
    TNFRSF8
  • Target description
    TNF receptor superfamily member 8
  • Target synonyms
    CD30, D1S166E, Ki-1, tumor necrosis factor receptor superfamily member 8, CD30L receptor, Ki-1 antigen, cytokine receptor CD30, lymphocyte activation antigen CD30
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP28908
  • Protein Name
    Tumor necrosis factor receptor superfamily member 8
  • Scientific Description
    Synthetic Peptide
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD30/TNFRSF8 using anti-CD30/TNFRSF8 antibody (A01225-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates, Lane 2: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD30/TNFRSF8 antigen affinity purified polyclonal antibody (Catalog # A01225-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD30/TNFRSF8 at approximately 120KD. The expected band size for CD30/TNFRSF8 is at 64KD.
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