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Immunohistochemical staining of human caudate shows strong nuclear and cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human caudate shows strong nuclear and cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human caudate shows strong nuclear and cytoplasmic positivity in neuronal cells.

Anti-TNK2 Antibody

HPA041954
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTNK2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TNK2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10188
  • Target name
    TNK2
  • Target description
    tyrosine kinase non receptor 2
  • Target synonyms
    ACK; ACK1; ACK-1; activated CDC42 kinase 1; activated Cdc42-associated kinase 1; activated p21cdc42Hs kinase; p21cdc42Hs; tyrosine kinase non-receptor protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ07912
  • Protein Name
    Activated CDC42 kinase 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ACK1/TNK2 using anti-ACK1/TNK2 antibody (A02334-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACK1/TNK2 antigen affinity purified polyclonal antibody (Catalog # A02334-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACK1/TNK2 at approximately 115 kDa. The expected band size for ACK1/TNK2 is at 115 kDa.
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