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Immunofluorescent staining of human cell line U2OS shows localization to vesicles.
Immunofluorescent staining of human cell line U2OS shows localization to vesicles.
Immunofluorescent staining of human cell line U2OS shows localization to vesicles.

Anti-TNXB Antibody

HPA016017
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetTNXB
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TNXB
  • Delivery Days Customer
    12
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7148
  • Target name
    TNXB
  • Target description
    tenascin XB
  • Target synonyms
    EDS3; EDSCLL; EDSCLL1; growth-inhibiting protein 45; hexabrachion-like protein; HXBL; tenascin XB1; tenascin XB2; tenascin-X; TENX; TNX; TN-X; TNXB1; TNXB2; TNXBS; VUR8; XB; XBS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22105
  • Protein Name
    Tenascin-X
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TNXB using anti-TNXB antibody (PB9833). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: MCF-7 Whole Cell Lysate, Lane 2: SW620 Whole Cell Lysate, Lane 3: HELA Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNXB antigen affinity purified polyclonal antibody (Catalog # PB9833) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNXB at approximately 458 kDa. The expected band size for TNXB is at 458 kDa.
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