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Tenascin X Recombinant Antibody

BSM-60611R
Bioss
ApplicationsImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTNXB
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Overview

  • Supplier
    Bioss
  • Product Name
    Tenascin X Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P(1:200-400), IHC-F(1:100-500), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    A1C11
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7148
  • Target name
    TNXB
  • Target description
    tenascin XB
  • Target synonyms
    EDS3, EDSCLL, EDSCLL1, HXBL, TENX, TN-X, TNX, TNXB1, TNXB2, TNXBS, VUR8, XB, XBS, tenascin-X, growth-inhibiting protein 45, hexabrachion-like protein, tenascin XB1, tenascin XB2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22105
  • Protein Name
    Tenascin-X
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNXB using anti-TNXB antibody (PB9833). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: MCF-7 Whole Cell Lysate, Lane 2: SW620 Whole Cell Lysate, Lane 3: HELA Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNXB antigen affinity purified polyclonal antibody (Catalog # PB9833) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNXB at approximately 458 kDa. The expected band size for TNXB is at 458 kDa.
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