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Immunohistochemical staining of human cerebral cortex shows weak granular positivity in cytoplasm in neurons.
Immunohistochemical staining of human cerebral cortex shows weak granular positivity in cytoplasm in neurons.
Immunohistochemical staining of human cerebral cortex shows weak granular positivity in cytoplasm in neurons.

Anti-TOMM40 Antibody

HPA036231
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetTOMM40
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TOMM40
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10452
  • Target name
    TOMM40
  • Target description
    translocase of outer mitochondrial membrane 40
  • Target synonyms
    C19orf1; D19S1177E; mitochondrial import receptor subunit TOM40 homolog; mitochondrial outer membrane protein; p38.5; PEREC1; PER-EC1; protein Haymaker; TOM40; translocase of outer membrane 40 kDa subunit homolog; translocase of outer mitochondrial membrane 40 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO96008
  • Protein Name
    Mitochondrial import receptor subunit TOM40 homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TOMM40 Polyclonal Antibody, Unconjugated (bs-17227R) at 1:200 overnight at 4°C, DAB staining.
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Figure 1. Western blot analysis of TOMM40 using anti-TOMM40 antibody (A03166-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOMM40 antigen affinity purified polyclonal antibody (Catalog # A03166-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOMM40 at approximately 38 kDa. The expected band size for TOMM40 is at 38 kDa.
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