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TOMM40 / TOM40 Antibody

LS-C332428
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetTOMM40
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TOMM40 / TOM40 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 34kDa/37kDa, while the observed MW by Western blot was 38kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 34kDa/37kDa, while the observed MW by Western blot was 38kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10452
  • Target name
    TOMM40
  • Target description
    translocase of outer mitochondrial membrane 40
  • Target synonyms
    C19orf1; D19S1177E; mitochondrial import receptor subunit TOM40 homolog; mitochondrial outer membrane protein; p38.5; PEREC1; PER-EC1; protein Haymaker; TOM40; translocase of outer membrane 40 kDa subunit homolog; translocase of outer mitochondrial membrane 40 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TOMM40 using anti-TOMM40 antibody (A03166-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOMM40 antigen affinity purified polyclonal antibody (Catalog # A03166-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOMM40 at approximately 38 kDa. The expected band size for TOMM40 is at 38 kDa.
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