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Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in granular pattern in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in granular pattern in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in granular pattern in exocrine glandular cells.

Anti-TRAF3 Antibody

HPA002933
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTRAF3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TRAF3 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7187
  • Target name
    TRAF3
  • Target description
    TNF receptor associated factor 3
  • Target synonyms
    CAP-1, CAP1, CD40bp, CRAF1, IIAE5, IMD132A, IMD132B, LAP1, RNF118, TNF receptor-associated factor 3, CD40 associated protein 1, CD40 binding protein, CD40 receptor associated factor 1, LMP1-associated protein 1, RING-type E3 ubiquitin transferase TRAF3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13114
  • Protein Name
    TNF receptor-associated factor 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of TRAF3 using anti-TRAF3 antibody (A00424). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat PC-12 cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse HEPA1-6 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF3 antigen affinity purified polyclonal antibody (Catalog # A00424) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF3 at approximately 60,64KD. The expected band size for TRAF3 is at 64KD.
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