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Human Jurkat cells probed with TRAF3 Polyclonal Antibody, Unconjugated (bs-1185R) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Human Jurkat cells probed with TRAF3 Polyclonal Antibody, Unconjugated (bs-1185R) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Human Jurkat cells probed with TRAF3 Polyclonal Antibody, Unconjugated (bs-1185R) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

TRAF3 Polyclonal Antibody

BS-1185R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTRAF3
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    TRAF3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7187
  • Target name
    TRAF3
  • Target description
    TNF receptor associated factor 3
  • Target synonyms
    CAP1; CAP-1; CD40 associated protein 1; CD40 binding protein; CD40 receptor associated factor 1; CD40bp; CRAF1; IIAE5; LAP1; LMP1-associated protein 1; RING-type E3 ubiquitin transferase TRAF3; RNF118; TNF receptor-associated factor 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13114
  • Protein Name
    TNF receptor-associated factor 3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of TRAF3 using anti-TRAF3 antibody (A00424). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat PC-12 cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse HEPA1-6 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF3 antigen affinity purified polyclonal antibody (Catalog # A00424) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF3 at approximately 60,64KD. The expected band size for TRAF3 is at 64KD.
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