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ProductsBack/Anti-Transcription factor RFX3 RFX3 Antibody
Western Blot (WB) analysis of specific cells using RFX3 Polyclonal antibody.
Western Blot (WB) analysis of specific cells using RFX3 Polyclonal antibody.
Western Blot (WB) analysis of specific cells using RFX3 Polyclonal antibody.

Anti-Transcription factor RFX3 RFX3 Antibody

A07674
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetRFX3
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Transcription factor RFX3 RFX3 Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID5991
  • Target name
    RFX3
  • Target description
    regulatory factor X3
  • Target synonyms
    transcription factor RFX3, DNA binding protein RFX3, regulatory factor X, 3 (influences HLA class II expression)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48380
  • Protein Name
    Transcription factor RFX3
  • Scientific Description
    Boster Bio Anti-Transcription factor RFX3 RFX3 Antibody catalog # A07674. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RFX3 using anti-RFX3 antibody (A07674-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFX3 antigen affinity purified polyclonal antibody (Catalog # A07674-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFX3 at approximately 95 kDa. The expected band size for RFX3 is at 84 kDa.
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