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Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.

Anti-TREX1 Antibody

HPA035437
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTREX1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TREX1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11277
  • Target name
    TREX1
  • Target description
    three prime repair exonuclease 1
  • Target synonyms
    AGS1, CRV, DRN3, HERNS, RVCLS, three-prime repair exonuclease 1, 3' repair exonuclease 1, 3'-5' exonuclease TREX1, DNase III, deoxyribonuclease III
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NSU2
  • Protein Name
    Three-prime repair exonuclease 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of TREX1 using anti-TREX1 antibody (PB9748). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: SMMC Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TREX1 antigen affinity purified polyclonal antibody (Catalog # PB9748) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TREX1 at approximately 33 kDa. The expected band size for TREX1 is at 39 kDa.
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